rat specific enzyme linked immunosorbent assay elisa kit  (Novus Biologicals)

Journal: Applied Microbiology and Biotechnology

Article Title: Investigating soil and ATCC bacterial strains for their ability to synthesize anisotropic gold nanoparticles

doi: 10.1007/s00253-025-13689-7

Figure Lengend Snippet: TEM images of the synthesized AuNPs under Gram-positive bacteria a – c Streptomyces sp. 11, d – f Streptomyces sp. 28, g – i Dietzia sp . , j – l E. faecalis ATCC, m – o S. aureus ATCC, and p – r L. acidophilus ATCC

Article Snippet: Fig. 4 TEM images of synthesized AuNPs under different representative Gram-negative and Gram-positive bacteria. a – b Elizabethkingia sp., c – d Comamonas sp., e – f Enterococcus sp., g – h Streptomyces sp. 36, i – j S. algae ATCC, and k SAED pattern image of AuNPs Fig. 5 TEM images of the synthesized AuNPs under Gram-positive bacteria a – c Streptomyces sp. 11, d – f Streptomyces sp. 28, g – i Dietzia sp ., j – l E. faecalis ATCC, m – o S. aureus ATCC, and p – r L. acidophilus ATCC Fig. 6 TEM images of synthesized AuNPs under Gram-negative bacteria a – c Pseudomonas sp. 26, d – f Pseudomonas sp. 27, g – i Pseudomonas sp. 25, j – l Aeromonas sp., m – o E. coli ATCC, and p – r R. palustris ATCC

Techniques: Synthesized, Bacteria

Journal: Applied Microbiology and Biotechnology

Article Title: Investigating soil and ATCC bacterial strains for their ability to synthesize anisotropic gold nanoparticles

doi: 10.1007/s00253-025-13689-7

Figure Lengend Snippet: Images of bacterial strains isolated from soil. Culture on agar plates and Gram stain. A Gram-positive bacteria, (a1-2) strain no. 11 ( Streptomyces sp.), (b1-2) strain no. 28 ( Streptomyces sp.), (c1-2) strain no. 36 ( Streptomyces sp.), (d1-2) strain no. 19 ( Dietzia sp . ) , and (e1-2) strain no. 35 ( Enterococcus sp.). B Gram-negative bacteria, (a1-2) strain no. 24 ( Elizabethkingia sp.), (b1-2) strain no. 25 ( Pseudomonas sp.), (c1-2) strain no. 26 ( Pseudomonas sp.), (d1-2) strain no. 27 ( Pseudomonas sp.), (e1-2) strain no. 33 ( Comamonas sp.), and (f1-2) strain no. 34 ( Aeromonas sp.)

Article Snippet: Fig. 3 Representative UV–vis results of AuNPs synthesized from DS-AuCl 4 by Gram-positive a Streptomyces sp. 36, b Enterococcus sp., and Gram-negative bacteria, c Elizabethkingia sp., d Comamonas sp., and e S. algae ATCC .

Techniques: Isolation, Staining, Bacteria

Journal: Applied Microbiology and Biotechnology

Article Title: Investigating soil and ATCC bacterial strains for their ability to synthesize anisotropic gold nanoparticles

doi: 10.1007/s00253-025-13689-7

Figure Lengend Snippet: Representative UV–vis results of AuNPs synthesized from DS-AuCl 4 by Gram-positive a Streptomyces sp. 36, b Enterococcus sp., and Gram-negative bacteria, c Elizabethkingia sp., d Comamonas sp., and e S. algae ATCC . Bacterial cell suspensions of 6.0 × 10 8 CFU/mL were incubated with 0.5 mM DS-AuCl 4 at 28 °C and 37 °C . Inset images of the test tubes displaying the development of color in the graph

Article Snippet: Fig. 3 Representative UV–vis results of AuNPs synthesized from DS-AuCl 4 by Gram-positive a Streptomyces sp. 36, b Enterococcus sp., and Gram-negative bacteria, c Elizabethkingia sp., d Comamonas sp., and e S. algae ATCC .

Techniques: Synthesized, Bacteria, Algae, Incubation

Journal: Applied Microbiology and Biotechnology

Article Title: Investigating soil and ATCC bacterial strains for their ability to synthesize anisotropic gold nanoparticles

doi: 10.1007/s00253-025-13689-7

Figure Lengend Snippet: TEM images of synthesized AuNPs under different representative Gram-negative and Gram-positive bacteria. a – b Elizabethkingia sp., c – d Comamonas sp., e – f Enterococcus sp., g – h Streptomyces sp. 36, i – j S. algae ATCC, and k SAED pattern image of AuNPs

Article Snippet: Fig. 3 Representative UV–vis results of AuNPs synthesized from DS-AuCl 4 by Gram-positive a Streptomyces sp. 36, b Enterococcus sp., and Gram-negative bacteria, c Elizabethkingia sp., d Comamonas sp., and e S. algae ATCC .

Techniques: Synthesized, Bacteria, Algae

Journal: Applied Microbiology and Biotechnology

Article Title: Investigating soil and ATCC bacterial strains for their ability to synthesize anisotropic gold nanoparticles

doi: 10.1007/s00253-025-13689-7

Figure Lengend Snippet: XPS analysis of AuNPs deposited using different bacterial species, Streptomyces sp. 36, Elizabethkingia sp., Comamonas sp., and S. algae ATCC. a survey spectra of AuNPs, b narrow scan of the Au 4f region, c narrow scan of the C 1 s region, and d narrow scan of the N 1 s region

Article Snippet: Fig. 3 Representative UV–vis results of AuNPs synthesized from DS-AuCl 4 by Gram-positive a Streptomyces sp. 36, b Enterococcus sp., and Gram-negative bacteria, c Elizabethkingia sp., d Comamonas sp., and e S. algae ATCC .

Techniques: Algae

Journal: Journal of Neuroinflammation

Article Title: Basophils activate splenic B cells and dendritic cells via IL-13 signaling in acute traumatic brain injury

doi: 10.1186/s12974-025-03621-1

Figure Lengend Snippet: Chemoattraction of basophils upon TBI occurs through CXCL1 expression in B-cells and DCs. A Principal component analysis (PCA) of the cytokine array revealed distinct clustering of the sham and TBI treatment groups. N = 5. B The heatmap of the cytokine array showed the significantly altered cytokines/chemokines in the spleen 3 h after TBI. N = 5. C Principal component analysis (PCA) of the vascular factors array revealed distinct clustering of the sham and TBI treatment groups. N = 6. D The heatmap of the vascular factors array showed the significantly altered cytokines/chemokines in the spleen 3 h after TBI. N = 6. E - H ELISA assays verification, in a larger subset of samples, showed the significant upregulation of CXCL1 (Sham vs TBI: 2642±1403 pg/ml vs 4439±1451 pg/ml; p = 0.0090; t = 2.893) and CCL6 (Sham vs TBI: 19466±3209 pg/ml vs 26175±6681 pg/ml; p = 0.0083; t = 2.916), a significant downregulation of IL-5 (Sham vs TBI: 1146±637 pg/ml vs 654±337 pg/ml; p = 0.0331; t = 2.298) and no difference in CXCL13 in the spleen. Plasma samples showed the significant upregulation only in CXCL1 (Sham vs TBI: 116±92 pg/ml vs 408±264 pg/ml; p = 0.0018; t = 3.387), with no differences observed in CXCL13, CCL6 and IL-5. Sham N = 10; TBI N = 13. I - L Single mRNA RNAscope revealed the significant increase of CXCL1 mRNA puncta in the whole spleen (Sham vs TBI: 47±7 vs 59±9; p = 0.0377; t = 2.488), as well as an increase of CXCL1+/CD19+ (magenta dotted outline; sham vs TBI: 11±4% vs 23±6%; p = 0.0033; t = 4.125) and CXCL1+/CD11c+ (yellow dotted outline; sham vs TBI: 15±4% vs 28±3%; p = 0.0006; t = 5.484) cells after TBI. N = 5. scale bar overview: 50 µm; scale bar insert: 10 µm. Data is shown as mean±SD. *: p < 0.05, **: p <0.01, ***: p <0.001.

Article Snippet: The ELISA assays for CXCL1, CXCL13, CCL6 and IL-5 on spleen and plasma were performed according to manufacturer’s instructions (mouse DuoSet ELISA; R&D systems).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Clinical Proteomics, RNAscope

Journal: Journal of Neuroinflammation

Article Title: Basophils activate splenic B cells and dendritic cells via IL-13 signaling in acute traumatic brain injury

doi: 10.1186/s12974-025-03621-1

Figure Lengend Snippet: Ethanol pretreatment prevents the chemotaxis of basophils to the spleen. A , B Immunofluorescence staining of spleen sections with CD19 and MCPT8 show a significant increase in MCPT8+ cells (white dotted outline) post TBI in the germinal center (SS vs ST: 8±1 vs 18±4; p = 0.0047) and marginal zone (SS vs ST: 11±1 vs 27±4; p < 0.0001) of splenic follicles. With a significant decrease in MCPT8+ cells with ethanol pretreatment, in both the germinal center (ST vs ET: 18±4 vs 8±3; p = 0.0032) as well as the marginal zone (ST vs ET: 27±4 vs 14±4; p = 0.0002). N = 4, scale bar overview: 100 µm; scale bar insert: 20 µm. C , D Immunofluorescence staining of spleen sections with CD19, CD11c, MCPT8 and pIL-13Ra1 showed the significant increase post TBI in pIL-13Ra1+/CD19+ (magenta dotted outline; SS vs ST: 22±4% vs 36±8%; p = 0.0328), pIL-13Ra1+/CD11c+ (yellow dotted outline; SS vs ST: 18±3% vs 27±5%; p = 0.0043) and pIL-13Ra1+/MCPT8+ (green dotted outline; SS vs ST: 11±3% vs 18±3%; p = 0.0154). With a significant decrease with ethanol pretreatment, in pIL-13Ra1+/CD19+ (ST vs ET: 36±8% vs 21±7%; p = 0.0146), pIL-13Ra1+/CD11c+ (ST vs ET: 27±5% vs 19±4%; p = 0.0119) and pIL-13Ra1+/MCPT8+ (ST vs ET: 18±3% vs 11±3%; p = 0.0176). N = 4, scale bar overview: 50 µm; scale bar insert: 20 µm. E. ELISA assay in a larger cohort of the 4 treatment groups (SS; ES; ST and ET) revealed a significant upregulation of CXCL1 post TBI (SS vs ST: 642±1403 pg/ml vs 4439±1451 pg/ml; p = 0.0066), with a significant decrease with ethanol pretreatment (ST vs ET: 4439±1451 pg/ml vs 2987±1145 pg/ml; p = 0.0303). SS N = 10; ES N = 7; ST N = 13; ET N = 9. Data is shown as mean±SD. *: p < 0.05, **: p <0.01.

Article Snippet: The ELISA assays for CXCL1, CXCL13, CCL6 and IL-5 on spleen and plasma were performed according to manufacturer’s instructions (mouse DuoSet ELISA; R&D systems).

Techniques: Chemotaxis Assay, Immunofluorescence, Staining, Enzyme-linked Immunosorbent Assay